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IGVFFI2046GKAD

released
validated
File Set
IGVFDS2972VXMB(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep2-BinD-PCRrep7_R2
md5sum
eaa164606ec1a30a8ef2e060dd04b637
Content MD5sum
b870f8a27d1439266cb2f07f0982fdc1
File Size
3.6 MB
Submitted File Name
230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep2-BinD-PCRrep7_S373_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R2
Index
TTGAGCCT:TGAC
Average Read Length
150
Read Count
92589

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford