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IGVFFI2032UXJP

released
validated
File Set
IGVFDS3945FAEL(gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
R2 reads from sequencing run 3
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispra-grna-rep3-rep3-fastq-lane1-R2
md5sum
9964db4589a6c0f1d4bae256306ede12
Content MD5sum
4af115bb8cb416e8c569c6c53a8fb492
File Size
2.5 GB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/gRNA_CRISPRa_D3_S12_L001_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NovaSeq 6000
Sequencing Run
3
Flowcell ID
HMCCWDSX3
Lane
1
Sequencing Kit
NovaSeq 6000 S4 Reagent Kit v1.5
Illumina Read Type
R2
Average Read Length
90
Read Count
65767381

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tar
filtered feature barcode matrix
gene by gene expression by CRISPR guide capture in filtered feature barcode matrix
Charles Gersbach, Duke
336 MB
validated

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI1328IASZ, IGVFFI2032UXJP
Charles Gersbach, Duke
1.1 KB
validated

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke