IGVFFI2004HRIY

released

validated

File Set

IGVFDS6541EVXL(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR3_Rep1_S40

md5sum

86bdc9b40155b37397ef0ca03dfa1255

Content MD5sum

f8cf827ec8a7b5f60ebd46d39093d51f

File Size

37 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinD-PCR3-Rep1_S40_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CCAGAGCT:TGCG

Average Read Length

Read Count

1922432

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9894YYOA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library