IGVFFI2002GGIA

released

validated

File Set

IGVFDS5130AKAZ(low FACS signal CRISPR knockout FACS screen sorted on the expression of IL7R integrating a guide (sgRNA) library targeting transcription start sites in many genes)

Summary

R1 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

charles-gersbach:cd8-tcells-measurement-set-crispr-ko-tfome-low-bin-rep1-R1

md5sum

5396b667b191f20217b3e84da87c26f5

Content MD5sum

cd237e8590c95acaee4e143e86856aac

File Size

800 MB

Submitted File Name

/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/pSM73_L2_S2_L001_R1_001.fastq.gz

Checkfiles Version

Legacy version of checkfiles

Sequencing Details

Sequencing Platform

Illumina MiSeq

Sequencing Run

Flowcell ID

AACJJ7LM5

Lane

Sequencing Kit

MiSeq Reagent Kit v2

Illumina Read Type

Average Read Length

Read Count

45527974

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4936HKEW

PrimaryCell

CD8-positive, alpha-beta memory T cell

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)