IGVFFI1999WHVY

released

validated

File Set

IGVFDS3559YNWI(low FACS signal CRISPR interference FACS screen sorted on the expression of B2M integrating a guide (sgRNA) library targeting transcription start sites in B2M)

Summary

R1 reads from sequencing run 2

File Format

fastq

Content Type

reads

Aliases

charles-gersbach:cd8-tcells-measurement-set-crispri-b2m-low-bin-rep2-R1

md5sum

aecfac79205a8f0bcb692ea49438547b

Content MD5sum

94af47014f5706d81e2a7cfb0401576d

File Size

7.1 MB

Submitted File Name

/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/B2M-Low-R2_S7_L001_R1_001.fastq.gz

Checkfiles Version

Legacy version of checkfiles

Sequencing Details

Sequencing Platform

Illumina MiSeq

Sequencing Run

Flowcell ID

000000000-J9HPF

Lane

Sequencing Kit

MiSeq Reagent Kit v2

Illumina Read Type

Average Read Length

Read Count

288441

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3427LEEW

PrimaryCell

CD8-positive, alpha-beta memory T cell

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)