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INDEXED
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SequenceFile
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IGVFFI1996TPSI
IGVFFI1996TPSI
released
validated
Json view
File Set
IGVFDS8535CXVX
(scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jay-shendure:CRISPRa_singlecell_K562_VP64_sequence_exprs_10Xlane2_seqlane1_I1
External Resources
SRA:SRR30585981
md5sum
dad0f9f95e77088ddab1354a6311148e
Content MD5sum
c24612cfe6f0f681c510e1fdeb5a2468
File Size
735 MB
Submitted File Name
/net/shendure/vol10/www/content/members/CRISPRa_QTL_website/public/data/K562_data/sequencing_data/cellranger_mkfastq_output/outs/fastq_path/AAAHG2HHV/VP64_exprs_2/VP64_exprs_2_S2_L001_I1_001.fastq.gz
Sequencing Details
Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAAHG2HHV
Lane
1
Sequencing Kit
NextSeq 1000/2000 P3 Reagent Kit
Illumina Read Type
I1
Average Read Length
8
Read Count
106910228
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM9390JSDH
InVitroSystem
K562
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library
Files Derived From This File
3 items
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
IGVFFI4612KEHH
csv
differential gene expression quantifications
differential gene expression quantifications
Jay Shendure, UW
3.3 KB
validated
IGVFFI5652WMRK
csv
differential gene expression quantifications
differential gene expression quantifications
Jay Shendure, UW
6.9 KB
validated
IGVFFI5731ZKRJ
tar
sparse gene count matrix
cell by gene in sparse gene count matrix
Jay Shendure, UW
538 MB
validated
Attribution
Award
HG011966
Principal Investigator(s)
Jay Shendure
,
Nadav Ahituv
,
Martin Kircher
Contact P.I.
Jay Shendure
Lab
Jay Shendure, UW