IGVFFI1935HBGJ

released

validated

File Set

IGVFDS7919SMDO(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF1-BinC-PCR4-Rep1_S171

md5sum

59275de6f2577a6ee714bcbb555f7bf8

Content MD5sum

bf7177c351794de0fe522b1cbad44b5a

File Size

31 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF1-BinC-PCR4-Rep1_S171_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TGTCGGAT:ATTG

Average Read Length

Read Count

1448084

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3123SZYY

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library