{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI1890UWCK/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI1890UWCK",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep3-FFrep1-BinE-PCRrep1_I2"
],
"assay_titles": [
"Variant-EFFECTS"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "f35b67a3e6b1c1fac33b26892da10804",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:07:21.588707+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/measurement-sets/IGVFDS6214RDRM/",
"accession": "IGVFDS6214RDRM",
"file_set_type": "experimental data",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM6445TGKO/",
"accession": "IGVFSM6445TGKO",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released",
"summary": "CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0001253/",
"status": "released",
"term_name": "THP-1"
}
],
"status": "released",
"summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 80-90% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "released",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus"
},
"file_size": 2762263,
"gene_list_for": [],
"href": "/sequence-files/IGVFFI1890UWCK/@@download/IGVFFI1890UWCK.fastq.gz",
"illumina_read_type": "I2",
"index": "CGTTACCA:ACTG",
"input_file_for": [
"/tabular-files/IGVFFI2325NRPY/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"maximum_read_length": 4,
"md5sum": "8a3caac6be94fee195c2068b0dc529ee",
"mean_read_length": 4,
"minimum_read_length": 4,
"read_count": 360657,
"release_timestamp": "2023-12-20T23:34:25.325613+00:00",
"s3_uri": "s3://igvf-files/2023/12/05/851317d8-c866-4c4b-8b51-e2b3cbb9539d/IGVFFI1890UWCK.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"sequencing_run": 2,
"status": "released",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_file_name": "220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep1-FFRep1-BinE-PCRrep1_S5_I2_001.fastq.gz",
"summary": "I2 reads from sequencing run 2",
"upload_status": "validated",
"uuid": "851317d8-c866-4c4b-8b51-e2b3cbb9539d"
}