IGVFFI1885EORP

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS1434CFNM(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-BinD-PCRRep2_R1
md5sum
fa56942972f793638fad693082393e90
Content MD5sum
cd4e1d6c6625a9d373b832ea3476977a
File Size
5.7 MB
Submitted File Name
Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-PCRRep2-BinD_S32_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
R1
Index
GCCGTCGA:TTGT
Average Read Length
150
Read Count
192660

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
variant effects
Jesse Engreitz, Stanford
5.9 KB
validated

Attribution