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IGVFFI1822KONB

released
validated
File Set
IGVFDS9813VHFD(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispri-gex-rep1-rep1-fastq-lane1-R2
md5sum
bf65c1a6ad77812e38c0e9b6f2fa041a
Content MD5sum
3d8d34063c99abceef2363f79bfd2a9b
File Size
18 GB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/GEX_CRISPRi_D1_S1_L001_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NovaSeq 6000
Sequencing Run
1
Lane
1
Sequencing Kit
NovaSeq 6000 S4 Reagent Kit v1.5
Illumina Read Type
R2
Average Read Length
90
Read Count
349280689

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tar
filtered feature barcode matrix
gene by gene expression by CRISPR guide capture in filtered feature barcode matrix
Charles Gersbach, Duke
383 MB
validated

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI1822KONB, IGVFFI2739KXTR
Charles Gersbach, Duke
981 B
validated

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke