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IGVFFI1799FWCW

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS9718OGWQ(cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW288C_1_R1
md5sum
716aeb0b401f76204753684b769f60a3
Content MD5sum
ebafd5a999ea1f9a122c97df02e82ee8
File Size
304 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-11-03-McDermott-NextSeq.10X.run2.10X/2023-11-03/outs/fastq_path/AAF3JCVM5//LW288C_S7_L001_R1_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Gary Hon, UT

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAF3JCVM5
Lane
1
Sequencing Kit
NextSeq 1000/2000 P3 Reagent Kit
Illumina Read Type
R1
Average Read Length
28
Read Count
15063903

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW288) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
589 MB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
429 MB
validated