IGVFFI1796ZBYH

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS3270YULL(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

R1 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep2-BinD-PCRRep7_R1

md5sum

216cdf724f442b05210ad0a8836451ee

Content MD5sum

0a76fd4d193fba790115e93464dfaf84

File Size

6.9 MB

Submitted File Name

Jurkat_Stim-IL2RA_prom-BioRep2-FFRep2-PCRRep7-BinD_S164_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

Illumina Read Type

Index

CGCCTTCC:GGCA

Average Read Length

150

Read Count

253631

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4553KVCE

InVitroSystem

Jurkat

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library