IGVFFI1790WPVC

released

validated

File Set

IGVFDS8557UXEJ(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF2-BinA-PCR3-Rep1_S175

md5sum

6a689724df10c760e9801ec63c09acbd

Content MD5sum

949829541e1a00e28ed1f92c9e3bcbad

File Size

29 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF2-BinA-PCR3-Rep1_S175_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GAAGAAGT:ATTG

Average Read Length

Read Count

1354935

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1718NCDJ

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library