IGVFFI1781AOFZ

released

validated

File Set

IGVFDS2965IRTU(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin0hrs-FF1-BinD-PCR1-Rep1_S220

md5sum

0b3db9029cf51a2273b23da0428a68e6

Content MD5sum

298599811d1304067b529f29871fbe05

File Size

24 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin0hrs-FF1-BinD-PCR1-Rep1_S220_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TAAGCACA:TTGA

Average Read Length

Read Count

1092558

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM6400EVKX

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library