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IGVFFI1762JZKE

released
validated
File Set
IGVFDS5374LMRK(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep1-FFrep3-BinD-PCRrep2_R2
md5sum
86e26a9a36c2dac3dbed808e921d252a
Content MD5sum
d4cf46d07da2e6f8e34732d3fbe4ede2
File Size
76 MB
Submitted File Name
220228pPPIF-FFSample-4-D21-pPPIF-Amp-pPPIF-1-3-2-BinD_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R2
Index
ATTGTCTG:TGAC
Average Read Length
150
Read Count
1960107

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford