IGVFFI1665CADL

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI1665CADL/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI1665CADL",
    "aliases": [
        "gary-hon:LW283B-3_1_I2"
    ],
    "assay_titles": [
        "Perturb-seq"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG011996/",
        "component": "functional characterization"
    },
    "content_md5sum": "c767c0f44f0276b24766cfda6a6e9c21",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2024-03-01T17:21:44.098772+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/auxiliary-sets/IGVFDS4262EBDF/",
        "accession": "IGVFDS4262EBDF",
        "file_set_type": "gRNA sequencing",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM8464JMJS/",
                "accession": "IGVFSM8464JMJS",
                "classifications": [
                    "differentiated cell specimen"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
                        "modality": "interference",
                        "status": "preview",
                        "summary": "CRISPRi Sp-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0009747/",
                        "status": "released",
                        "term_name": "GM25256 (WTC-11)"
                    }
                ],
                "status": "preview",
                "summary": "GM25256 (WTC-11) differentiated cell specimen induced to neural stem cell for 20 days (cellular sub pool: subpool_LW283), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
                "targeted_sample_term": {
                    "@id": "/sample-terms/CL_0000047/",
                    "status": "preview",
                    "term_name": "neural stem cell"
                },
                "taxa": "Homo sapiens"
            }
        ],
        "status": "preview",
        "summary": "gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
    },
    "file_size": 208578277,
    "flowcell_id": "AAC7VLGHV",
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI1665CADL/@@download/IGVFFI1665CADL.fastq.gz",
    "illumina_read_type": "I2",
    "input_file_for": [],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/gary-hon/",
        "title": "Gary Hon, UT"
    },
    "lane": 1,
    "loci_list_for": [],
    "maximum_read_length": 10,
    "md5sum": "8ce8d083989cf06d27157afb0e86a28e",
    "mean_read_length": 10,
    "minimum_read_length": 10,
    "read_count": 27650781,
    "s3_uri": "s3://igvf-files/2024/03/01/2d5ed479-154e-4dda-8e1c-02a1e8c11e5c/IGVFFI1665CADL.fastq.gz",
    "schema_version": "14",
    "seqspecs": [],
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0010963/",
        "status": "released",
        "term_name": "Illumina NextSeq 2000"
    },
    "sequencing_run": 1,
    "status": "preview",
    "submitted_by": {
        "@id": "/users/b05645f1-2d56-4a5e-b272-4601e555a3fa/",
        "title": "Mpathi Nzima"
    },
    "submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-10-05-McDermott-NextSeq.10X/2023-10-05/outs/fastq_path/AAC7VLGHV/LW283B-3_S29_L001_I2_001.fastq.gz",
    "summary": "I2 reads from sequencing run 1",
    "upload_status": "validated",
    "uuid": "2d5ed479-154e-4dda-8e1c-02a1e8c11e5c"
}