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IGVFFI1639KPBO

released
validated
File Set
IGVFDS9275AQHE(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep1-FFrep1-BinD-PCRrep7_I2
md5sum
aeeb033a38b1cb6e25a30bf5da900736
Content MD5sum
bb3db3a4df047b37f65eab7b965ab272
File Size
2.4 MB
Submitted File Name
230523pPPIFEnformerTHP1-THP1-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep1_BinD-PCRrep7_S43_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I2
Index
TAAGCACA:CAGT
Average Read Length
4
Read Count
271680

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford