IGVFFI1545RRLD

released

validated

File Set

IGVFDS2834VAYS(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinB-PCR2_Rep2_S87

md5sum

95b476a09a91e4c4331156c7ffbb7b89

Content MD5sum

f2dacd9dce388c27444c07b7553a0f8c

File Size

28 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinB-PCR2-Rep2_S87_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTCTAGG:TCTC

Average Read Length

Read Count

1450087

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2313EONT

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library