IGVFFI1527KBMM

released

validated

File Set

IGVFDS3113JRZB(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin0hrs-FF2-BinF-PCR1-Rep1_S234

md5sum

08e5d0e71c1ac3da778c5b8c962554f1

Content MD5sum

56e702e95f16a32c802eb11100ef03bb

File Size

21 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin0hrs-FF2-BinF-PCR1-Rep1_S234_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CTAACTCG:TTGA

Average Read Length

Read Count

976510

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM6932DXSV

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library