IGVFFI1480XPQY

released

validated

File Set

IGVFDS1409CHZY(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF2-BinB-PCR4_S116

md5sum

41075a85f96b5b2a0c10783f7d636301

Content MD5sum

8a9764874277c905acc7b86324c9a009

File Size

22 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF2-BinB-PCR4_S116_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CAGGAGCC:ATTG

Average Read Length

Read Count

900972

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3263KXLP

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library