IGVFFI1476GXSD

released

validated

File Set

IGVFDS8375WKPF(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinD-PCR3_S268

md5sum

99b6d556a5b5294aeb4ae58015036a65

Content MD5sum

049a4145fc9760ef141fdcb16b49057f

File Size

10 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin0hrs-FF1-BinD-PCR3_S268_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TCGCTAGA:GTAC

Average Read Length

Read Count

435941

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4919VWFX

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library