IGVFFI1472PMJU

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS3617RUHJ(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep1-BinC-PCRrep3_R2
md5sum
d8365f62d8b9f2dfc56d8239eec4a9ae
Content MD5sum
e7d2857c490b755e25b2265d8777a590
File Size
9.2 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_C12-Amp_PPIF_5splicesite-BioRep1-FFrep1-BinC-PCRrep3_S901_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
R2
Index
GACCTAAC:TATGAGTA
Average Read Length
151
Read Count
421357

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution