IGVFFI1421XINF

released

validated

File Set

IGVFDS2059NFJA(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinE-PCR3_Rep1_S41

md5sum

5ddf80cf599472276e5ce0d1b1d1cd23

Content MD5sum

825874ed4ae7aa6529c6bb5e46716d92

File Size

38 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinE-PCR3-Rep1_S41_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TCCTTGGT:TGCG

Average Read Length

Read Count

1951900

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1124JJAA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library