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IGVFFI1421DDQV

released
validated
File Set
IGVFDS5533XJRU(gRNA sequencing for mixed CRISPR TAP-seq integrating a guide (sgRNA) library targeting accessible genome regions in 29 genomic loci)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:auxiliary_files_CRUDO_TAPseq-Auxin6hrs-Rep1_S3_R2
md5sum
82e573d7fe6ef2d7724ba99e69f26f86
Content MD5sum
d5dc29ef87f038f6707eca637e546b64
File Size
415 MB
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/221125_Cohesin_TAPseq_v1/221125_Cohesin_TAPseq_v1/outs/fastq_path/L363_S4_L003_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Lane
3
Illumina Read Type
R2
Average Read Length
90
Read Count
9579210

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI4105QVFH, IGVFFI8910PMMH, IGVFFI1421DDQV
Jesse Engreitz, Stanford
1.2 KB
validated

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford