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IGVFFI1408GFJL

released
validated
File Set
IGVFDS7083JDYN(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep2-BinD-PCRrep5_I2
md5sum
f86e2f682fcb1e35e9846d12cd8ab7f0
Content MD5sum
0bdb08a07e465944c5b542b9699bbe6b
File Size
830 KB
Submitted File Name
230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep2-BinD-PCRrep5_S89_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I2
Index
CCAGAGCT:CAGT
Average Read Length
4
Read Count
95453

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford