IGVFFI1373KUKR

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS9743TYOT(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

R1 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep1-BinB-PCRRep2_R1

md5sum

f97e5970767d2747f11e69ca383ec9ba

Content MD5sum

847b3b9f483db6979aadd2cda518dabd

File Size

6.0 MB

Submitted File Name

Jurkat_Stim-IL2RA_prom-BioRep2-FFRep1-PCRRep2-BinB_S38_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

Illumina Read Type

Index

TTCGCTGA:TTGT

Average Read Length

150

Read Count

204315

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1469NQHM

InVitroSystem

Jurkat

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library