IGVFFI1365SKHU

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI1365SKHU/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI1365SKHU",
    "aliases": [
        "gary-hon:LW269C_1_R2"
    ],
    "assay_titles": [
        "Perturb-seq"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG011996/",
        "component": "functional characterization"
    },
    "content_md5sum": "cb63ba37ab0013df9ef5db973ad0bd6b",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2024-04-25T22:51:45.713488+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/auxiliary-sets/IGVFDS5720GLEJ/",
        "accession": "IGVFDS5720GLEJ",
        "file_set_type": "cell hashing barcode sequencing",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM9223FAYO/",
                "accession": "IGVFSM9223FAYO",
                "classifications": [
                    "differentiated cell specimen"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
                        "modality": "interference",
                        "status": "preview",
                        "summary": "CRISPRi Sp-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0003045/",
                        "status": "released",
                        "term_name": "H9"
                    }
                ],
                "status": "preview",
                "summary": "H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW269), female, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
                "targeted_sample_term": {
                    "@id": "/sample-terms/CL_0000746/",
                    "status": "released",
                    "term_name": "cardiac muscle cell"
                },
                "taxa": "Homo sapiens"
            }
        ],
        "status": "preview",
        "summary": "cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
    },
    "file_size": 1326263542,
    "flowcell_id": "AAF5CKNM5",
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI1365SKHU/@@download/IGVFFI1365SKHU.fastq.gz",
    "illumina_read_type": "R2",
    "input_file_for": [],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/gary-hon/",
        "title": "Gary Hon, UT"
    },
    "lane": 1,
    "loci_list_for": [],
    "maximum_read_length": 90,
    "md5sum": "6c99817c63cf87c60155c34de4416b0a",
    "mean_read_length": 90,
    "minimum_read_length": 90,
    "read_count": 33042956,
    "s3_uri": "s3://igvf-files/2024/04/25/6d91188c-6f33-4853-8579-84da1cb75313/IGVFFI1365SKHU.fastq.gz",
    "schema_version": "14",
    "seqspecs": [],
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0010963/",
        "status": "released",
        "term_name": "Illumina NextSeq 2000"
    },
    "sequencing_run": 2,
    "status": "preview",
    "submitted_by": {
        "@id": "/users/b05645f1-2d56-4a5e-b272-4601e555a3fa/",
        "title": "Mpathi Nzima"
    },
    "submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-12-22-McDermott-NextSeq.10X/2023-12-22/outs/fastq_path/AAF5CKNM5//LW269C_S3_L001_R2_001.fastq.gz",
    "summary": "R2 reads from sequencing run 2",
    "upload_status": "validated",
    "uuid": "6d91188c-6f33-4853-8579-84da1cb75313"
}