{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI1303ZTTN/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI1303ZTTN",
"aliases": [
"jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep3-BinD-PCRrep5_R2"
],
"assay_titles": [
"Variant-EFFECTS"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "e41ee4bba2b328f40f8cffcdebce4a64",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-13T10:53:07.115204+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/measurement-sets/IGVFDS5913KHIA/",
"accession": "IGVFDS5913KHIA",
"file_set_type": "experimental data",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM7851LWAO/",
"accession": "IGVFSM7851LWAO",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released",
"summary": "CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"status": "released",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "released",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus"
},
"file_size": 12376113,
"gene_list_for": [],
"href": "/sequence-files/IGVFFI1303ZTTN/@@download/IGVFFI1303ZTTN.fastq.gz",
"illumina_read_type": "R2",
"index": "TTGAATAG:GTCA",
"input_file_for": [
"/tabular-files/IGVFFI8402CAPM/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"maximum_read_length": 150,
"md5sum": "b22d45d195d31e8f8ad14edcd9ab4294",
"mean_read_length": 150,
"minimum_read_length": 150,
"read_count": 292173,
"release_timestamp": "2023-12-21T01:00:14.105153+00:00",
"s3_uri": "s3://igvf-files/2023/12/13/4f3078f0-db63-49cf-8d0d-3b4183bf3506/IGVFFI1303ZTTN.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"sequencing_run": 1,
"status": "released",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep3_BinD-PCRrep5_S137_R2_001.fastq.gz",
"summary": "R2 reads from sequencing run 1",
"upload_status": "validated",
"uuid": "4f3078f0-db63-49cf-8d0d-3b4183bf3506"
}