IGVFFI1262UZWR

released
validated
File Set
IGVFDS3673BXYO(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF3-BinE-PCR1_S29
md5sum
b93f720de5df6b844b35019469da7a8b
Content MD5sum
006666c7cc0bcd75dd2ddce390f84c6d
File Size
8.1 MB
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF3-BinE-PCR1_S29_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Index
TCGCCTTG:CTCT
Average Read Length
21
Read Count
335325

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI1262UZWR
Jesse Engreitz, Stanford
903 B
validated

Attribution