IGVFFI1257KXEP

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI1257KXEP/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI1257KXEP",
    "aliases": [
        "jesse-engreitz:sequence_files-TeloHAEC_Perturb-seq_2kG-Dialout_2kg_2PMDox1_4_redo_GAAGAAGT_L001_R1_001.fastq.gz"
    ],
    "assay_titles": [
        "Perturb-seq"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization"
    },
    "content_md5sum": "5dcf298a63bfb6611cf38296249413c3",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2024-02-06T21:21:28.558576+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/auxiliary-sets/IGVFDS7065BOZB/",
        "accession": "IGVFDS7065BOZB",
        "file_set_type": "gRNA sequencing",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM3700IHHL/",
                "accession": "IGVFSM3700IHHL",
                "classifications": [
                    "cell line"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
                        "modality": "interference",
                        "status": "released",
                        "summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/NTR_0000753/",
                        "status": "released",
                        "term_name": "TeloHAEC"
                    }
                ],
                "status": "preview",
                "summary": "TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
                "taxa": "Homo sapiens"
            }
        ],
        "status": "preview",
        "summary": "gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci"
    },
    "file_size": 335378213,
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI1257KXEP/@@download/IGVFFI1257KXEP.fastq.gz",
    "illumina_read_type": "R1",
    "input_file_for": [],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "loci_list_for": [],
    "maximum_read_length": 28,
    "md5sum": "c7062ae25890363fe819337a2e4eb431",
    "mean_read_length": 28,
    "minimum_read_length": 28,
    "read_count": 16005573,
    "s3_uri": "s3://igvf-files/2024/02/06/d405a295-9bc0-4ca6-9a50-d3a23912c5a7/IGVFFI1257KXEP.fastq.gz",
    "schema_version": "14",
    "seqspecs": [],
    "sequencing_kit": "NextSeq 500/550 High-Output v2.5 Kit",
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0009173/",
        "status": "released",
        "term_name": "Illumina NextSeq 500"
    },
    "sequencing_run": 1,
    "status": "preview",
    "submitted_by": {
        "@id": "/users/05fa0dc9-0e33-427c-9d1f-82393edc4a03/",
        "title": "Helen Kang"
    },
    "submitted_file_name": "/oak/stanford/groups/engreitz/Users/gschnitz/dialout_files_for_helen/Dialout_2kg_2PMDox1_4_redo_GAAGAAGT_L001_R1_001.fastq.gz",
    "summary": "R1 reads from sequencing run 1",
    "upload_status": "validated",
    "uuid": "d405a295-9bc0-4ca6-9a50-d3a23912c5a7"
}