IGVFFI1233YJYR

released

validated

File Set

IGVFDS6678UHIV(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF2-BinD-PCR1_Rep2_S193

md5sum

adee05edf5a4bd170e1db1518a62ac83

Content MD5sum

73a1aa79623480eb15700d4f9ac20cd4

File Size

22 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF2-BinD-PCR1-Rep2_S193_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CCTATGCC:GCCA

Average Read Length

Read Count

1126961

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4390QYVS

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library