IGVFFI1203ZTZE

released

validated

File Set

IGVFDS8375WKPF(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinD-PCR1_S76

md5sum

7a9425695c6785d0ea1fa7bdc0977145

Content MD5sum

409f1853f3e096ab3e7f30f11b6ec77f

File Size

7.4 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin0hrs-FF1-BinD-PCR1_S76_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TCGCTAGA:ACGT

Average Read Length

Read Count

314704

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4919VWFX

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library