IGVFFI1193HDXG

released

validated

File Set

IGVFDS3914IPWH(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinB-PCR4_Rep2_S185

md5sum

350cac0a5f8131eeccc4129b3edb4908

Content MD5sum

99f8bc277e6a84cda186b1259f31e6c8

File Size

15 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF1-BinB-PCR4-Rep2_S185_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CAGGAGCC:GCCA

Average Read Length

Read Count

777124

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0361IFQU

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library