IGVFFI1150JCHT

released

validated

File Set

IGVFDS6281UOJN(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF3-BinF-PCR1_S30

md5sum

73d6ddab75cdeb9cc28a40b3cbe4337c

Content MD5sum

72f74a0fccdbebe7bc7481271a3f8ab4

File Size

13 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF3-BinF-PCR1_S30_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATAGCGTC:CTCT

Average Read Length

Read Count

533935

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7141IWGF

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library