IGVFFI1148OPQE

released
validated
File Set
IGVFDS1861LEUZ(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep4-FFrep1-BinC-PCRrep1_I1
md5sum
d587690ed1ed8efbd75a8e6be0c23d1c
Content MD5sum
85e4ac1cf286d10395b4ca62e9944ac9
File Size
3.2 MB
Submitted File Name
220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S19-BioRep4-FFRep1-BinC-PCRrep1_S157_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
I1
Index
CTATGCGT:GTCA
Average Read Length
8
Read Count
404071

Samples

1 item
Accession
Type
Sample Terms
Summary
In Vitro Systems
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 35-45% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution