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IGVFFI1139IHLH

released
validated
File Set
IGVFDS6512WYQI(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep2-FFrep1-BinA-PCRrep4_I1
md5sum
7c53433344b5ffb3874a3af7151b1f22
Content MD5sum
f400e925cbd46aec8f3d0348258bd5e4
File Size
1.6 MB
Submitted File Name
230807S022-THP1-P153-Amp_PPIF_promoter-BioRep2-FFrep1-BinA-PCRrep4_S196_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I1
Index
GAAGAAGT:ACTG
Average Read Length
8
Read Count
180115

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford