IGVFFI1136TXZO

released

validated

File Set

IGVFDS6689ALTU(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF3-BinA-PCR3_S121

md5sum

15d1f0fd1d6223dcbc36a47cc8be7bf5

Content MD5sum

c16ff30ae64da85d472cd02c38484c11

File Size

28 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF3-BinA-PCR3_S121_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GAAGAAGT:ATTG

Average Read Length

Read Count

1188547

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2914OOTJ

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library