IGVFFI1121YBEV

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS4537KNTB(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

R2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep2-BinB-PCRRep3_R2

md5sum

dd669fd8cbc30c65db20a524c95672f0

Content MD5sum

6a2dd6ee7f497379488f5497e0d33088

File Size

6.6 MB

Submitted File Name

Jurkat_Stim-IL2RA_prom-BioRep2-FFRep2-PCRRep3-BinB_S66_R2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

Illumina Read Type

Index

ATTGTCTG:TTGT

Average Read Length

150

Read Count

208152

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3690RUXJ

InVitroSystem

Jurkat

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library