IGVFFI1103OELL

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS3215JVHK(cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW430C_6_R1
md5sum
3aa0746c394f3bf337d90a6a7bdfef36
Content MD5sum
518665cea0ed99faf51c6309afab9d27
File Size
4.5 GB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/HTO//LW430C_S24_L006_R1_001.fastq.gz
Checkfiles Version
Attribution

Sequencing Details

Sequencing Platform
Sequencing Run
1
Flowcell ID
227C7WLT4
Lane
6
Sequencing Kit
NovaSeq X Series 25B Reagent Kit
Illumina Read Type
R1
Average Read Length
151
Read Count
72298882

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW430) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
1.0 GB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
768 MB
validated