Home

IGVFFI1100RGNN

released
validated
File Set
IGVFDS8801DBGC(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep2-BinB-PCRrep1_R1
md5sum
f91ec43c6edfb238bfe46f6fe330256c
Content MD5sum
c711d1a9247132ab9e4462b2a46114b7
File Size
7.9 MB
Submitted File Name
230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep2-BinB-PCRrep1_S61_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R1
Index
AGTTGCTT:GTCA
Average Read Length
150
Read Count
295004

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford