{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI1100OAWK/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI1100OAWK",
"aliases": [
"jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep1-FFrep4-BinD-PCRrep3_R1"
],
"assay_titles": [
"Variant-EFFECTS"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "7641d04310fb5ecea1ac8a9b6bcda166",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-11-26T06:58:55.907666+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/measurement-sets/IGVFDS5030BFVD/",
"accession": "IGVFDS5030BFVD",
"file_set_type": "experimental data",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM1648EHGD/",
"accession": "IGVFSM1648EHGD",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released",
"summary": "CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0001253/",
"status": "released",
"term_name": "THP-1"
}
],
"status": "preview",
"summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "preview",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus"
},
"file_size": 15183387,
"gene_list_for": [],
"href": "/sequence-files/IGVFFI1100OAWK/@@download/IGVFFI1100OAWK.fastq.gz",
"illumina_read_type": "R1",
"index": "TACTTAGC:CGCTATGT",
"input_file_for": [
"/tabular-files/IGVFFI6212XMWZ/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"maximum_read_length": 151,
"md5sum": "77c40d81cdac3743108f29793cc4b285",
"mean_read_length": 151,
"minimum_read_length": 151,
"read_count": 870156,
"s3_uri": "s3://igvf-files/2024/11/26/9695e2b4-080a-43df-bba7-fdbba1dfb491/IGVFFI1100OAWK.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_platform": {
"@id": "/platform-terms/EFO_0022840/",
"status": "released",
"term_name": "Illumina NovaSeq X"
},
"sequencing_run": 1,
"status": "preview",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_file_name": "240426S024-Enhancer-THP1-PPIF_Enhancer_val_guides-Amp_PPIF_enhancer-BioRep1-FFrep4-BinD-PCRrep3_S597_R1_001.fastq.gz",
"summary": "R1 reads from sequencing run 1",
"upload_status": "validated",
"uuid": "9695e2b4-080a-43df-bba7-fdbba1dfb491"
}