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IGVFFI1042XEFF

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS5699EGGK(cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW427C_6_I1
md5sum
98978c9f8182db595ac058e78b1e2f49
Content MD5sum
e7d0c25afedb426043fb0a1acacf3b19
File Size
596 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/HTO//LW427C_S21_L006_I1_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles

Sequencing Details

Sequencing Platform
Illumina NovaSeq 6000
Sequencing Run
1
Flowcell ID
227C7WLT4
Lane
6
Sequencing Kit
NovaSeq X Series 25B Reagent Kit
Illumina Read Type
I1
Average Read Length
10
Read Count
76950029

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW427), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
1.0 GB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
768 MB
validated

Attribution

Award
HG011996
Principal Investigator(s)
Contact P.I.
Gary Hon
Lab
Gary Hon, UT