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IGVFFI1022LUTK
IGVFFI1022LUTK
released
validated
Json view
File Set
IGVFDS7395DPAD
(scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jay-shendure:CRISPRa_singlecell_K562_VPR_sequence_exprs_10Xlane1_seqlane2_R1
External Resources
SRA:SRR30585980
md5sum
077cd167bedf81449e8a284ca42af0ff
Content MD5sum
9fda28f55d57436fdebeda36cf44978f
File Size
2.5 GB
Submitted File Name
/net/shendure/vol10/www/content/members/CRISPRa_QTL_website/public/data/K562_data/sequencing_data/cellranger_mkfastq_output/outs/fastq_path/AAAHG2HHV/VPR_exprs_1/VPR_exprs_1_S3_L002_R1_001.fastq.gz
Sequencing Details
Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAAHG2HHV
Lane
2
Sequencing Kit
NextSeq 1000/2000 P3 Reagent Kit
Illumina Read Type
R1
Average Read Length
28
Read Count
118556932
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM1594ZOQH
InVitroSystem
K562
K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library
Files Derived From This File
3 items
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
IGVFFI4612KEHH
csv
differential gene expression quantifications
differential gene expression quantifications
Jay Shendure, UW
3.3 KB
validated
IGVFFI5652WMRK
csv
differential gene expression quantifications
differential gene expression quantifications
Jay Shendure, UW
6.9 KB
validated
IGVFFI5731ZKRJ
tar
sparse gene count matrix
cell by gene in sparse gene count matrix
Jay Shendure, UW
538 MB
validated
Attribution
Award
HG011966
Principal Investigator(s)
Jay Shendure
,
Nadav Ahituv
,
Martin Kircher
Contact P.I.
Jay Shendure
Lab
Jay Shendure, UW