IGVFFI1019WGYS

released

validated

File Set

IGVFDS1369IMLU(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin0hrs-FF1-BinF-PCR1-Rep1_S6

md5sum

ee739778b22ee6412f51c46ee05841c8

Content MD5sum

06a89b10a00b4cf4eabcfd949d058d95

File Size

29 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin0hrs-FF1-BinF-PCR1-Rep1_S6_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AGTTGCTT:TTGA

Average Read Length

Read Count

1351841

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4326ZGNE

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library