IGVFFI1011WVNT

released

validated

File Set

IGVFDS3352ZNFQ(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinA-PCR3_S265

md5sum

8ddc8cbfaf07ba8533cccabac44ab21a

Content MD5sum

ab0b15c6f3681c1fec760dc419f1818d

File Size

6.4 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin0hrs-FF1-BinA-PCR3_S265_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AACAATGG:GTAC

Average Read Length

Read Count

271485

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7865RIGH

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library