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IGVFFI1008SZVT

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS2136RFQE(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW234B-2_1_R2
md5sum
884946e3c21651a8ea2524d0f65d0feb
Content MD5sum
1ff89e95aa8d8b8bb0c4e6d27d579d81
File Size
1.4 GB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-03-24-McDermott-NextSeq.10X/2023-03-24/outs/fastq_path/AAC235NHV/LW234B-2_S4_L001_R2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAC235NHV
Lane
1
Illumina Read Type
R2
Average Read Length
90
Read Count
49959674

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW264), male, Homo sapiens modified with CRISPRi Sp-dCas9, transfected with a guide library

Files Derived From This File

2 items
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
783 MB
validated
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
4.5 GB
validated

Attribution

Award
HG011996
Principal Investigator(s)
Contact P.I.
Gary Hon
Lab
Gary Hon, UT