IGVFFI1004HOZK

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS1434CFNM(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

I2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-BinD-PCRRep3_I2

md5sum

3ad6166200a0def375436de3579b008f

Content MD5sum

4cc8eb6e24119e5a2318b0c626afffd7

File Size

3.2 MB

Submitted File Name

Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-PCRRep3-BinD_S56_I2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

Illumina Read Type

Index

ACTAAGAC:TTGT

Average Read Length

Read Count

404807

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9448WLVQ

InVitroSystem

Jurkat

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library