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IGVFFI0982GCGH

released
validated
File Set
IGVFDS6229XAIE(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep3-BinD-PCRrep12_R1
md5sum
733378b63f2642d86b3f76a2ff9230f6
Content MD5sum
e99ac6d0a6eeeb147d1768c28fa5ecb1
File Size
3.1 MB
Submitted File Name
230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep3-BinD-PCRrep12_S336_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R1
Index
TTAATCAG:TGAC
Average Read Length
150
Read Count
98996

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford