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IGVFFI0955GZSP

released
validated
File Set
IGVFDS8504HJPW(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep1-BinA-PCRrep2_I2
md5sum
7edb6c71612a9fabfc81c9e3a1af6c55
Content MD5sum
a823d5f09178b9d33e1ce54935577b9e
File Size
834 KB
Submitted File Name
230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep1-BinA-PCRrep2_S194_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I2
Index
ACCAACTG:ACTG
Average Read Length
4
Read Count
98736

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford