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IGVFFI0952BGMF

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS8110KLML(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW424_4_I2
md5sum
33fb83e6e653085a6d62edac1512be90
Content MD5sum
95a52bf1cdcbbe448a032d30b7ec766f
File Size
2.6 GB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon17_18/scRNA//LW424_S2_L004_I2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles

Sequencing Details

Sequencing Platform
Illumina NovaSeq X Plus
Sequencing Run
1
Flowcell ID
227C7WLT4
Lane
4
Sequencing Kit
NovaSeq X Series 25B Reagent Kit
Illumina Read Type
I2
Average Read Length
10
Read Count
381572858

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW424), female, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
1.9 GB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
513 MB
validated

Attribution

Award
HG011996
Principal Investigator(s)
Contact P.I.
Gary Hon
Lab
Gary Hon, UT